西安凯新生物科技有限公司是国内ling命科学和生物技术领域试剂供应商，从成立初期就致力于为生命科学和生物技术领域提供产品和技术服务，力求将的产品和服务奉献给我们的客户。目前主要产品有：合成磷脂、血清、高分子聚乙二醇衍生物、嵌段共聚物、磁性纳米颗粒、纳米金及纳米金棒、近红外荧光染料、活性荧光染料、荧光标记的葡聚糖BSA和链霉亲和素、蛋白交联剂、小分子PEG衍生物、点计化学产品、树枝状聚合物、环糊精衍生物、热门肿瘤原料药、大环配体类、荧光量子点、透明质酸衍生物、石墨烯或氧化石墨烯、碳纳米管、富勒烯等等，可以满足从毫克级到公斤级的定制需求。我们非常乐于倾听您的需求，并一如既往地继续为您提供的产品和服务。
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产品简介：
DBCO (Dibenzocyclooctyne) is a cycloalkyne that can reacts with azides via strain-promoted 1,3-dipolar cycloaddition in aqueous solution, a bioorthogonal reaction also termed Cu-free click reaction. This reaction has excellent selectivity and biocompatibility, such that the complimentary reagents can form covalent bonds with richly functionalized biological systems. The Cu-free click reaction has been a powerful tool in catalyst-free bioconjugation. DBCO reagents posses fast kinetcs and stability in aqueous buffer and they can be used to label azide-modified biomolecules with high specificity and reactivity.
Physical Properties:
Yellow/pale-yellow solid or semi-solid;
Soluble in DMSO or DMF;
Storage Conditions:
Store at -20 0C, desiccate. Avoid frequent thaw and frozen.
Copper-free Click Reaction Procedures:
Prepare the azide-containing sample in reaction buffer. Add DBCO conjugate to azide containing sample. Recommendation: Add 1 mole equivalent of limiting reagent to 1.5-3.0 mole equivalents of highest abundance reagent. Incubate the reaction at room temperature for 2-4 hours or 2-12 hours at 4 degree celcius. The reaction is now ready for purification.
