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>技术文章>Rabbit Anti-Urease β
技术文章

Rabbit Anti-Urease β

阅读:189发布时间:2012-7-24

 

Rabbit Anti-Urease β
Catalog Number : HZ938123       Quantity size :   0.1ml (dilute with pH 7.4    0.01 M PBS or antibody diluent )
Background: Helicobacter pylori is present in the stomachs of at least half of the world’s population. Although gastic adenocarcinoma is associated with the presence of H. pylori in the stomach, only a small fraction of colonized individuals develop this common malignancy. H. pylori urease, an enzyme that generates ammonia and is present within the lamina propria of colonized individuals, binds to class II major histocompatability complex (MHC) molecules on the surfaces of gastric epithelial cells in vitro. In addition, Urease, which may be toxic to gastic epithelial cells, may also induce apoptosis. Specifically, Urease plays a crucial role in the development of ulcers in the duodenum by accelerating apoptosis in the antral mucosa. Ammonia accelerates TNFα cytokineinduced apoptosis, while ammonia or urease alone are unable to induce apoptosis. Urease exists as two forms, Urease α (UreA) and Urease β (UreB).
pecificity :   
        Anti-Urease β  is a rabbit polyclonal antibody unconjugated                
         specific for Urease β  of human, mouse and rat
         use for western blotting,elisa,immunoprecipitation and immunohistochemistry
         Protein G affinity chromatography purification, purity :>95%
         Isotype:  IgG
         mol wt:  61kDa
Rabbit Anti-Urease β
Application :  
        Western blotting 1:100-500                
         Immunohistochemistry 1:100-500                 
         ELISA 1:500-1000                  
         Optimal working dilutions must be determined by the end user.
Storage: Store at -20 for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody, the antibody is stable for at least six weeks at 2-4
 Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.

Rabbit Anti-Urease β


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