邇ｯ菫晏惠郤ｿ>莠ｧ蜩∝ｺ챴/a>> 逕溽黄隸募奄>逕溽黄譽豬玖ｯ募奄>蜈ｶ螳・函迚ｩ譽豬玖ｯ募奄>>驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華이/a>

驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華이a href="javascript:void(0)" onclick="addCompareForDetail(this)" data-id='24851723' data-classid='8707' data-name='驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華・ class="comBtn" target="_self">

蜿り쓈ｻｷ	髱｢隶ｮ

蜿り쓈ｻｷ

髱｢隶ｮ

蜈ｷ菴捺윞莠､莉ｷ莉･蜷亥酔蜊剰ｮｮ荳ｺ蜃열/div>

蜈ｬ蜿ｸ蜷咲ｧｰ菫｡蟶・函迚ｩ
蜩ƺnbsp; 迚죴span title="GEMIC">GEMIC
蝙ƺnbsp; 蜿ｷ
謇蝨ｨ蝨ｰ荳頑ｵｷ蟶쏴/span>
蜴ょ膚諤ｧ雍ｨ逕滉ｺｧ蜴ょｮｶ
譖ｴ譁ｰ譌ｶ髣ｴ2025/2/14 10:48:02
隶ｿ髣ｮ谺｡謨ｰ1168

莠ｧ蜩∵・ｭｾ:

驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2 荳榊ｸｦ譬・ｭｾ

蝨ｨ郤ｿ隸｢莉ｷ謾ｶ阯丈ｺｧ蜩썴/a> 霑帛콻螻募膚螻募床

閨皮ｳｻ譁ｹ蠑擾ｼ쿴b class="person">闔ｫ蟆丞ｧ쫴/b> 13764793648 譟･逵玖＃邉ｻ譁ｹ蠑쩴/a>

閨皮ｳｻ謌台ｻｬ譌ｶ隸ｷ隸ｴ譏取弍邇ｯ菫晏惠郤ｿ荳顔恚蛻ｰ逧안ｿ｡諱ｯ華瑚ｰ｢隹｢!

蜈ｬ蜿ｸ莉狗ｻ쥴/a> 荳ｻ關･莠ｧ蜩썴/a>

蜈ｳ莠取콻莉ｬ

菫｡蟶・函迚ｩ華梧弍荳螳ｶ髮ƺ應ｺｧ蜩∫泌書縲콻楳蜚ｮ縲∵橿譛ｯ譛榊苅"荳ｺ荳菴鍋噪逕溽黄蜈ｬ蜿ｸ縲らｻ剰ｿ콻､壼ｹｴ逧콻巻蜉帛書螻募ｷｲ謌蝉ｸｺ荳謎ｸ壻ｾ帛ｺ泌推邀ｻ逕溽黄遘醍皮畑蜩∫噪蜈ｬ蜿ｸ華碁柄譛滉ｸ捺ｳｨ莠守函迚ｩ鬚콻沺華梧覧荳倶ｻ｣逅왈ｺｧ蜩∫ｧ咲ｱｻ莨怜､壹ゆｿ｡蟶・函迚ｩ遘画価莉･莠ｧ蜩∬ｴｨ驥丈ｸｺ譬ｹ譛ｬ華御ｻ･蟶ょ惻髴豎ゆｸｺ蟇ｼ蜷托ｼ御ｻ･荳ｺ螳｢謌ｷ蛻幃莉ｷ蛟ｼ荳ｺ蟾ｱ莉ｻ逧・ｲｾ逾橸ｼ悟撓螳井ｺｧ蜩∬ｴｨ驥冗噪蜿ｯ髱縲∫ｨｳ螳夲ｼ悟ｹｶ荳･譬ｼ遘画価窶탴/span>荳謎ｸ壹∬ｯ壼ｮ槭∝ｮ井ｿ｡縲∝・譁ｰ " 蜈ｨ霑・ｨ九∝､壽婿菴咲噪雍ｨ驥冗尅謗ｧ譽豬具ｼ御ｻ主桁陬콻芦蜩∬ｴｨ華御ｻ惹ｺｧ蜩∝芦譛榊苅華梧ｯ丈ｸｪ邇ｯ闃ゑｼ梧콻莉ｬ驛ｽ荳･謗ｧ雍ｨ驥丞・華悟鴨豎ゆｸｺ螳｢謌ｷ謠蝉ｾ帶峩螂ｽ逧・∵峩荳謎ｸ壽ｧ逧ƺ橿譛ｯ譛榊苅蜥檎ｧ醍比ｺｧ蜩√よ콻莉ｬ萓帛ｺ皮噪莠ｧ蜩∝桁諡ｬ elisa 隸募奄逶抵ｼ檎函蛹冶ｯ募奄逶抵ｼ悟勘迚ｩ陦蛻ｶ蜩・ｼ梧蒲菴難ｼ瑚ｴｨ謗ｧ蜩・ｼ悟ｹｲ謇ｰ迚ｩ雍ｨ華梧蒲蜴滂ｼ檎函蛹冶ｯ募奄華悟ｮ樣ｪ瑚玲攝遲이/span>10 荳웈ｽ咏ｧ咲ｱｻ華悟얬蜿ｸ髟ｿ譛溯우蜉帑ｺ惹ｸｺ螳｢謌ｷ謠蝉ｾ클/span>RT-PCR 譽豬九∫羅逅ƺ沒濶ｲ縲∝콻逍ｫ扈콻喧譽豬九썴/span>Western blotting 譽豬九∫函蛹匁｣豬九썴/span>ELISA 譽豬九∵叛蜈肴｣豬狗ｭ我ｸ邉ｻ蛻怜ｮ樣ｪ御ｻ｣豬区恪蜉｡縲ゆｺｧ蜩∵ｶ牙所蛻콻ｭ千函迚ｩ蟄ｦ縲∫ｻ왊ƺ逕溽黄蟄ｦ縲∝콻逍ｫ蟄ｦ遲臥函蜻ｽ遘大ｭｦ逧콻推荳ｪ鬚콻沺縲쏴/span>

荳ｻ關･荳壼苅
荳ｻ關･莠ｧ蜩썴/span>: 雍ｨ謗ｧ蜩・ｼ悟ｹｲ謇ｰ迚ｩ雍ｨ華梧蒲菴捺蒲蜴滂ｼ죴/span>elisa 隸募奄逶抵ｼ悟勘迚ｩ陦蛻ｶ蜩・ｼ檎函蛹冶ｯ募奄逶堤ｭ峨ょｮ樣ｪ御ｻ｣蛛壽恪蜉｡華쿴/span>HE 譟楢牡 / 迚ｹ谿頑沒濶ｲ / 謚譛ｯ譛榊苅縲∝콻逍ｫ扈콻喧螳樣ｪ梧恪蜉｡縲썴/span>Western blotting 螳樣ｪ梧恪蜉｡縲썴span style="margin: 0px; padding: 0px; font-family: Verdana, sans-serif; color: rgb(51, 51, 51); background-image: initial; background-position: initial; background-size: initial; background-repeat: initial; background-attachment: initial; background-origin: initial; background-clip: initial;">RT-PCR 螳樣ｪ梧橿譛ｯ譛榊苅 ,QPCR( 闕ｧ蜈牙ｮ夐㍼ PCR 華峨콻ƺ閨泌콻逍ｫ ELISA 縲∫函蛹匁ｵ玖ｯ輔∵ｶｲ逶ｸ豌皮嶌譽豬九썴/span>RACE 縲∝얪蝓ｺ蝗蜷域윞縲∫ｺｿ邊剃ｽ捺ｵ句ｺ上∝渕蝗蜈矩嚀縲∝ｮ夂せ遯∝序縲∵沒濶ｲ菴捺ｭ･遘ｻ縲∝渕蝗扈콻얪蠎丞・闔ｷ蜿悶∫識蠅콻ｾｮ逕溽黄螟壽ｷ諤ｧ縲∝ｾｮ逕溽黄蝓ｺ蝗扈ƺｵ句ｺ上∝ｾｮ逕溽黄闖檎ｧ埼俺螳壹∫抜蝓ｺ蛹門ƺ譫先恪蜉｡遲峨らｻ城楳蜩∫煙華쿴/span>Abcam 縲썴/span>Sigma 縲썴/span>CST 縲썴/span>Amresco 縲썴/span>BD 縲썴/span>Enzo 縲썴/span>Bio-Rad 縲썴/span>merck 縲썴/span>Santa Cruz 縲썴/span>Jackson 縲썴/span>R&D 縲썴/span>Merck Millipore 縲썴/span>gibco 縲썴/span>hyclone 縲썴/span>Bio-World 縲썴/span>NEB 縲썴/span>Tocris 縲썴/span>Corning 縲썴/span>Axygen 遲臥ｭ峨쏴/span>

譬ｸ蠢쓈ｻｷ蛟ｼ

蜈ｬ蜿ｸ譛ｬ逹莉･荳謎ｸ夂噪莠ｧ蜩√∝粋逅・噪莉ｷ譬ｼ縲∝ｮ悟埋逧ƺ恪蜉｡華御ｸｺ螳｢謌ｷ謠蝉ｾ帶峩螂ｽ逧・ｼ梧峩荳謎ｸ夂噪謚譛ｯ譛榊苅蜥檎ｧ醍比ｺｧ蜩√ょ얬蜿ｸ莉･窶탴/span>雍ｨ驥丈ｸｺ驥搾ｼ御ｸ謎ｸ壽恪蜉｡ " 逧콻次蛻呻ｼ瑚우蜉帑ｺ惹ｸｺ逕ｨ謌ｷ謠蝉ｾ幃ｫ俶ｧ莉ｷ豈皮噪隸募奄逶貞柱譽豬区恪蜉｡華梧署萓帛얪遞句暢隸｢蜥梧橿譛ｯ謾ｯ謖・ｼ悟桁諡ｬ遑ｮ螳壽ｵ句ｮ壽欠譬・콻画叫蜷磯ら噪隸募奄逶定ｧƺｼ縲∬ｯ募奄逶剃ｿ晏ｭ倥콻｢ƺｵ句ｮ壼柱豬句ｮ夂ｻ捺棡蛻ƺ梵遲峨るｫ倩ｴｨ驥冗噪莠ｧ蜩・ｼ瑚ｮｩ逕ｨ謌ｷ逵∝ｿ・ｼ帑ｸ謎ｸ夂噪譽豬区恪蜉｡華瑚ｮｩ逕ｨ謌ｷ逵∝鴨華帑ｼ俶Β逧안ｻｷ譬ｼ華瑚ｮｩ逕ｨ謌ｷ逵콻蝶縲ゆｿ｡蟶・函迚ｩ荳ｺ蟷ｿ螟ｧ逕ｨ謌ｷ謠蝉ｾ帛推邀ｻ譽豬区恪蜉｡華悟ｸｮ蜉ｩ閠∝ｸ郁ｽｻ譚ｾ闔ｷ蠕嶺ｺ・悄螳槫庄髱逧ƺ｣豬区焚謐ｮ華梧仞闡玲署鬮倅ｺ・畑謌ｷ逧・ｧ醍疲譜邇・ｼ御ｸｺ蜈ｶ蜿題｡ｨ鬮俶ｰｴ蟷ｳ逧・皮ｩｶ隶ｺ譁ƺ署萓帑ｺ콻撓螳樒噪蟷ｳ蜿ｰ華帑ｸｺ遐皮ｩｶ逕滓潔譌ｶ豈穂ｸ夲ｼ悟ｯｼ蟶磯｡ｺ蛻ｩ扈馴｢倥∫筏隸ｷ譁ｰ隸ｾ鬚伜柱譎句合閨檎ｧｰ謠蝉ｾ帑ｺ콻ｮ槫ｮ槫惠蝨ｨ逧콻ｸｮ蜉ｩ華悟얬蜿ｸ螳｢謌ｷ驕榊ｸ콻嵜蜀콻推螟ｧ蟄ｦ縲∝現髯｢縲∫皮ｩｶ謇縲∝梱逕滄亟逍ｫ縲∫函迚ｩ蜈ｬ蜿ｸ遲牙黒菴阪쏴/span>

螻募ｼ譏ｾ遉ｺ譖ｴ螟壼콻螳ｹ

ELISA隸募奄逶抵ｼ檎函蛹冶ｯ募奄華梧蒲菴難ｼ梧콻㊥蜩콻

莠ｧ蜩∫ｮ莉존/a> 蝨ｨ郤ｿ隸｢莉ｷ

莠ｧ蜩∝錐遘ｰ華夐㍾扈ƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華이br>闍ｱ譁콻錐遘ｰ華啌ecombinant Core Streptavidin 2, r-cSA2
隗쓦譬ｼ華콻 mg華콻0 mg華콻00 mg華콻00 mg
郤ｯ蠎ｦ華壺翁95%華・DS-PAGE 譽豬具ｼ이br>譚･貅撰ｼ壼､ｧ閧譚왊曙蜿鷹콻蟾･遞玖曙譬ｪ
菫晏ｭ俶擅莉ｶ華콻20邃쓈ｿ晏ｭ컴br>譛읦謨웦譛滂ｼ콻 蟷ｴ
譛ｬ莠ｧ蜩∽ｻ었ｾ帷ｧ醍泌ｮ樣ｪ檎畑華御ｸ榊★蜈ｶ莉也畑騾斐콻

驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華이/span> 莠ｧ蜩∽ｿ｡諱ｯ

莠ｧ蜩∝錐遘ｰ華쿴/span> 驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2 華井ｸ榊ｸｦ譬・ｭｾ華이/span>

闍ｱ譁콻錐遘ｰ華쿴/span> Recombinant Core Streptavidin 2, r-cSA2 華이/span>

隗ƺnbsp;譬ｼ華쿴/span> 1 mg 華죴/span>10 mg 華죴/span>100 mg 華죴/span>500 mg

莠ｧ蜩∝ｽ｢蠑擾ｼ쿴/span> 蜀ｷ蜀ｻ蟷ｲ邊会ｼ웴/span>5mM 逎ｷ驟ｸ髓ｾ逶千ｼ灘ƺ豸ｲ縲∵ｵｷ阯ｻ邉厄ｼ죴/span>pH 8.0 華이/span>

蛻ƺnbsp;蟄ƺnbsp;驥擾ｼ쿴/span> 蝗幄★菴ƺnbsp; 53.2kDa 華悟黒菴ƺnbsp; 13.3kDa

豢ｻ諤ｧ華쿴/span> 竕･16 U/mg 陋狗區華웴/span>Green 謾ｹ濶ｯ豕墓ｵ句ｮ夲ｼ이/span>

郤ｯ蠎ｦ華쿴/span> 竕･95% 華웴/span>SDS-PAGE 譽豬具ｼ이/span>

豬灘ｺｦ豬句ｮ夲ｼ쿴/span> 邏ｫ螟門精謾ｶ豕包ｼ梧ｶ亥콻邉ｻ謨ｰ E(0.1% at 280 nm) =3.16

蜀ƺnbsp;豈ƺnbsp;邏華쿴/span> 竕､5EU/mg

譚･貅撰ｼ쿴/span> 螟ｧ閧譚왊曙蜿鷹콻蟾･遞玖曙譬ｪ

菫晏ｭ俶擅莉ｶ華쿴/span> -20邃쑴/span>菫晏ｭ컴/span>

譛ƺnbsp;謨ƺnbsp;譛滂ｼ쿴/span> 1 蟷ｴ

逶ｸ蜈ｳ莉狗ｻ搾ｼ쿴/span>

體ｾ髴我ｺｲ蜥檎ｴ華웴/span>Streptavidin 華죴/span>SA 華画弍髦ｿ扈ｴ荳콻得髴芽曙華・treptomyces avidinii華牙惠逕滄柄霑・ｨ倶ｸｭ蛻ƺｳ檎噪荳遘榊酔蝙句屁閨壻ｽ楢寞逋ｽ縲ょ酔莠ｲ蜥檎ｴ荳譬ｷ華御ｸ鞫ｩ蟆皮噪體ｾ髴我ｺｲ蜥檎ｴ蜿ｯ莉･扈灘粋蝗帶束蟆皮噪逕溽黄邏華御ｸ守函迚ｩ邏蜈ｷ譛牙ｾ磯ｫ倡噪莠ｲ蜥悟鴨縲ら罰莠짴/span>體ｾ髴我ｺｲ蜥檎ｴ荳榊性邉門渕荳皮ｭ臥鳩轤ｹ謗･霑台ｺ惹ｸｭ諤ｧ華悟屏豁､體ｾ髴我ｺｲ蜥檎ｴ蝨ｨ譽豬句ｺ皮畑荳ｭ蜈ｷ譛画ｯ比ｺｲ蜥檎ｴ譖ｴ菴守噪髱樒音蠑よｧ扈灘粋豌ｴ蟷ｳ縲る㍾扈ƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2 荳主､ｩ辟ｶ體ｾ髴我ｺｲ蜥檎ｴ逶ｸ豈費ｼ悟悉髯､荳取ｴｻ諤ｧ譌蜈ｳ逧콻ｺ丞・華御ｻ었ｿ晉蕗荳取ｴｻ諤ｧ譛牙・逧ƺｸ蠢콻ｺ丞・華檎ｨｳ螳壽ｧ縲∵ｺｶ隗｣諤ｧ遲画婿髱｢蝮웈ｼ倅ｺ主､ｩ辟ｶ體ｾ髴我ｺｲ蜥檎ｴ縲쏴/span>( 隸･驥咲ｻ콻得髴我ｺｲ蜥檎ｴ 2 pI 華쿴/span>6.09)

隸･蛻ｶ蜩∝ｷｲ陲ｫ蟷ｿ豕帛ｺ皮畑莠主､夂ｧ咲函迚ｩ謚譛ｯ鬚콻沺華瑚ｯｸ螯ゑｼ壼桁陲ｫ蜈咲稔譽豬狗畑蠕ｮ蟄疲攸華悟宛螟ƺnbsp; SA 蛛ｶ閨秘ƺ蛻ｶ蜑ゑｼ죴/span>SA 蛛ｶ閨碑懇蜈臥ｴ縲썴/span>SA 蛛ｶ閨皮｣∫匠遲会ｼ瑚ｿ幄悟盾荳朱ƺ閨泌콻逍ｫ蜷ｸ髯콻柱驟ｶ蛯ｬ蛹匁叛螟ｧ螳樣ｪ鯉ｼ悟콻逍ｫ扈콻喧蛹門ｭｦ縲ƺnbsp;逕溽黄蛻콻ｭ千ｺｯ蛹悶∫函迚ｩ莨諢溷勣縲∫函迚ｩ郤ｳ邀ｳ蠕ｮ逅・콻｢콻攜蜷大宛闕ｯ遐皮ｩｶ蜥檎函迚ｩ闃ｯ迚웊｢ｫ譁咏ｭ峨쏴/span>

菴ｿ逕ｨ譁ｹ豕包ｼ夲ｼ井ｻ었ｾ帛盾閠・ｼ이/span>

逕ｱ莠惹ｺｧ蜩∫函莠ｧ蟾･濶ｺ荳ｭ蜀ｻ蟷ｲ邇ｯ闃ゆｼ壼ｼ募콻菫晄侃蜑らｭ会ｼ御ｸｺ蜃丞ｰ第お逧콻ｮ樣ｪ瑚ｯｯ蟾ｮ華悟ｻｺ隶ｮ蜈磯夊ｿ콻콻驛ｨ譽豬区婿豕包ｼ御ｺｧ蜩∽ｸｭ陋狗區霑幄｡悟㊥遑ｮ螳夐㍼蜷趣ｼ悟콻霑幄｡悟錘扈ｭ螳樣ｪ鯉ｼ悟ｹｶ謖牙ｮ樣刔諠콻콻霑幄｡御ｺｧ蜩∫畑驥剰ｰƺ紛縲ょｻｺ隶ｮ莨伜얚騾画叫邏ｫ螟門精謾ｶ豕戊ｿ幄｡悟ｮ夐㍼華梧婿豕募ｦゆｸ具ｼ쿴/span>

蟆ƺｷ蜩∽ｻ惹ｽ取ｸｩ譚｡莉ｶ荳区響蜃ｺ蜷趣ｼ悟ｹｳ陦｡閾ｳ螳､貂ｩ華悟ｹｶ謫ｦ蜴ｻ逑ｶ螟夜刋逹逧콻・蜃晄ｰｴ華悟ｰ丞ｿƺ遠蠑逑ｶ逶厄ｼ碁亟豁｢蟷ｲ邊蛾｣樊ｺƺ此螟ｱ縲ら畑蜴ｻ遖ｻ蟄先ｰｴ謌也ｼ灘ƺ豸ｲ霑幄｡悟､肴ｺｶ華悟콻蛻ƺｺｶ隗｣蜷趣ｼ檎ｨ驥願・蜷磯よｵ灘ｺｦ華悟ｰ丞ｿ・ｧｻ蜈･豈碑牡逧ｿ荳ｭ華御ｻ･蜴ｻ遖ｻ蟄先ｰｴ謌也ｼ灘ƺ豸ｲ荳ｺ遨ｺ逋ｽ蟇ｹ辣ｧ華梧ｵ句ｮ壽ｷ蜩∝惠 280 nm 螟・噪蜷ｸ蜈牙ｼ華亥ｻｺ隶ｮ蜷ｸ蜈牙ｼ謗ｧ蛻ｶ蝨ｨ 0.1-1.5 蛹ｺ髣ｴ蜀・ｼ会ｼ悟ｰ콻ƺ莉｣蜈･莉･荳句얬蠑剰ｮ｡邂苓寞逋ｽ雍ｨ豬灘ｺｦ華쿴/span>

驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華ƺ/><span style=

萓句ｦょｰƺ悽莠ｧ蜩∝・蟷ｲ邊牙콻蛻ƺｺｶ隗｣蟷ｶ豺ｷ蛹蜷趣ｼ悟叙騾る㍼蜴滓ｶｲ遞驥ƺnbsp; 10 蛟搾ｼ梧ｵ句ｾ怜ƺ蝨ｨ 280 nm 螟・噪蜷ｸ蜈牙ｼ荳ｺ 0.948 華悟콻蜴滓ｶｲ逧않寞逋ｽ豬灘ｺｦ荳ｺ 10 ﾃ카/span>0.948/3.16=3 mg/mL 縲쏴/span>

豕ｨ諢丈ｺ矩｡ｹ華쿴/span>

1. 驥咲ｻ콻得髴我ｺｲ蜥檎ｴ 2 蜀ｻ蟷ｲ邊画・貅ｶ莠取ｰｴ華梧ｺｶ隗｣諤ｧ蜿ｯ霎ｾ 10 mg / mL 謌匁峩鬮假ｼ클/span>

2. 蟒ｺ隶ｮ菴ｿ逕ｨ郤ｯ豌ｴ貅ｶ隗｣華클/span>

3. 螯よ怏譛ｪ貅ｶ隗｣迚ｩ雍ｨ華悟ｻｺ隶ｮ蟒ｶ髟ｿ螟肴ｺｶ譌ｶ髣ｴ縲ゆｹ溷庄莉･騾夊ｿ・ｦｻ蠢ƺƺ蜈ｶ莉匁婿蠑城勁蜴ｻ荳肴ｺｶ迚ｩ蜷趣ｼ悟콻逕ｨ莠主錘扈ｭ螳樣ｪ鯉ｼ御ｸ堺ｼ壼ｯｹ諤ｻ陋狗區莠ｧ逕溷ｾ亥､ｧ逧콻ｽｱ蜩阪쏴/span>

4. 閠쓊剔蛻ｰ陋狗區雍ｨ迚ｹ谿頑ｧ華悟・蟷ｲ邊牙ｻｺ隶ｮ邇ｰ逕ｨ邇ｰ驟搾ｼ梧ｺｶ隗｣蜷主ｺ秘∩蜈榊渚螟榊・陞搾ｼ悟ｦる怙螟壽ｬ｡菴ｿ逕ｨ華悟ｻｺ隶ｮ謖蛾怙蛻왊｣콻錘蜀ｻ蟄假ｼ웴/span>-20 邃・ｼ会ｼ幃∩蜈榊惠 4 邃쓈ｸ矩柄譛溷ｭ俶叛縲쏴/span>

驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華ƺ/></span><strong><span style=

豕ｨ諢擾ｼ壽悽莠ｧ蜩∽ｻ콻剞莠守ｧ醍比ｽｿ逕ｨ縲쏴/span>

驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2 華井ｸ榊ｸｦ譬・ｭｾ華이/span>

蜈ｳ髞ｮ隸搾ｼ쿴a href="http://gsrxyx.com/chanpin-21449.html" target="_blank" style="color: #891e2a;text-decoration: underline;margin-right: 10px;">莨諢溷勣闃ｯ迚왦

逶ｸ蜈ｳ謚譛ｯ譁・ｫ >

閨壽ｰｨ驟ｯ蛻콻ｭ千ｭ帛惠閨壽ｰｨ驟ｯ菴鍋ｳｻ豺ｻ蜉驥쩴/a>

驥肴黒蜑ら噪蠑募콻蜿ｯ莉･騾夊ｿ웈ｸ､遘肴婿蠑丞ｮ퇴/a>

蜥ｨ隸｢荳ｭ蠢쑴/h3>
蝨ｨ郤ｿ螳｢譛쥴/span> QQ莠､隹웦QQ:1652493179

蟶ょ惻驛ｨ QQ莠､隹웦QQ:546130258

蜿大ｸ쓊ｯ｢莉ｷ蟒ｺ隶ｮ蜿埼ｦ웴/a>

蝗槫芦鬘ｶ驛ｨ

邇ｯ菫晏惠郤ｿ - 邇ｯ菫晁｡御ｸ壺應ｺ定＃鄂・窶晄恪蜉｡蟷ｳ蜿ｰ

蟇ｹ豈疲쩴a href="javascript:void(0);" target="_self">

豺ｻ蜉莠ｧ蜩썴/a>

蟇ｹ豈쳴/a> 貂・ｩｺ蟇ｹ豈疲쩴/a>

螂ｽ隸콻
荳ｭ隸콻
蟾ｮ隸콻

蛹ｿ蜷쥴/span>

謠蝉ｺ､

螳｢謌ｷ隸안ｻｷ蛻苓｡ｨ

隸안ｻｷ譌ｶ髣ｴ	邀ｻ蛻ｫ	隸ｦ扈콻콻螳ｹ	隸안ｻｷ閠앴/td>	IP蝨ｰ蝮

諤ｻ蜈ｱ譚｡譛ｬ鬘ｵ譚｡



閨皮ｳｻ譁ｹ蠑쩴/p>

菫｡蟶・函迚ｩ

13764793648

閨皮ｳｻ莠ｺ闔ｫ蟆丞ｧ쫴/div>

隶ｩ蜊門ｮｶ閨皮ｳｻ謌쭴/div>

蜥ｨ隸｢邀ｻ蝙具ｼ쿴/span>

隸｢莉ｷ莠ｧ蜩콻

蜚ｮ蜷取恪蜉｡

隸ｦ扈ƺ緒霑ｰ

隸ｷ邂蜊墓緒霑ｰ謔ｨ逧콻怙豎ゑｼ碁剞200蟄카/textarea>                     </div>                 </div> 				<div class="view-input-box">                     <span>蜊쵴b></b>菴쥴/span>                     <div class="input-box"><input type="text" name="" id="viewcompanyname" maxlength="30"><p class="Wrong" style="display: none;"></p></div>                 </div>                 <div class="view-input-box">                     <span>閨皮ｳｻ莠ｺ</span>                     <div class="input-box"><input type="text" name="" id="viewlinkname" maxlength="10"><p class="Wrong" style="display: none;"></p></div>                 </div>                 <div class="view-input-box">                     <span>逕ｵ<b></b>隸텴/span>                     <div class="input-box"><input type="text" name="" id="viewtel" maxlength="20"><p class="Wrong" style="display: none;"></p></div>                 </div>                 <div class="view-input-box view-code">                     <span>鬪瑚ｯ∫썴/span>                     <div class="input-box">                     <input type="text" name="viewcontactcode" id="viewcontactcode" maxlength="5"><img id="viewcodeimg" src="/ValidCode.aspx?type=viewcontact&v=1"                      onclick="this.src='/ValidCode.aspx?type=viewcontact&v='+Math.random()" title="轤ｹ蜃ｻ蛻ｷ譁ｰ鬪瑚ｯ∫ƺ style="cursor:pointer;">                      <p class="Wrong" style="display: none;"></p></div>                 </div> 				                <div class="view-btn"> 					                    <button onclick="SendCompanyContactMessage(146609)">謠蝉ｺ､菫｡諱ｯ</button>                     <div class="view-tip">謠蝉ｺ､蜷趣ｼ碁ｻ倩ｮ､謔ｨ蜷梧э<a href="/UserManage/login.aspx?agreement3" target="_blank">縲頑恪蜉｡譚｡谺ｾ縲존/a>蜥죴a href="/UserManage/login.aspx?agreement4" target="_blank">縲企嚼遘∝刻隶ｮ縲존/a></div>                 </div>             </div>         </div>     </div>    	<input type="hidden" id="viewtitle" value="驥咲ｻƺｸ蠢콻得髴我ｺｲ蜥檎ｴ 2華井ｸ榊ｸｦ譬・ｭｾ華ƺ  />     <input type="hidden" id="viewpid" value="24851723"  /> 		<div class="view-contact" id="view-contact-success" style="display:none">         <div class="view-guest-success">             <a href="javascript:" class="close" onclick="document.getElementById('view-contact-success').style.display='none';" target="_self"><i class="Public icon-P-cancel-fill"></i></a>             <div class="view-icon"><i class="success-icon"></i></div>             <div class="view-content">                 <div class="tip">謔ｨ逧・蕗險蟾ｲ謠蝉ｺ､謌仙粥~</div>                 <p>驥웊ｴｭ謌冶ｯ｢莉ｷ莠ｧ蜩・ｼ瑚ｯｷ逶ｴ謗･諡ｨ謇鍋鳩隸晁＃邉ｻ</p>                 <p>閨皮ｳｻ莠ｺ華夊辞蟆丞ｧ쫴/p>                 <div class="contact-us">                     <mark>                         <i class="use-icon"></i>                         <span>閨皮ｳｻ譁ｹ蠑ƺ<b id="viewcontentmobile"></b></span>                     </mark>                 </div>                 <div class="view-btn"><a href="javascript:" onclick="jQuery('#view-contact-success').hide();return false;" target="_self">謌醍衍驕謎ｺ열/a></div>             </div>         </div>     </div> 		<script type="text/javascript">         function ClearFristInfo(clint, info, stype) {             if (stype == 1) {                 if (document.getElementById(clint).value == info) {                     document.getElementById(clint).value = '';                 }             }             else if (document.getElementById(clint).value == "")                 document.getElementById(clint).value = info;         } 		 function ChktxtDetail() {         var txtdetail_ = jQuery.trim(jQuery("#txtDetail").val()); 		//console.log(txtdetail_)        // currObj = jQuery("#txtDetail");         if (txtdetail_.length != 0 && txtdetail_ != '隸ｷ邂蜊墓緒霑ｰ謔ｨ逧콻怙豎ゑｼ碁剞200蟄・) {             return true;         }         else {             //alert("隸ｷ邂蜊墓緒霑ｰ"); 			//jQuery("#txtDetail").focus();         }         return false;     }  //譏ｾ遉ｺ蜈ｬ蜿ｸ閨皮ｳｻ莠ｺ var _issendcontact=true; function ShowCompanyContact(Cid) {     jQuery("#viewcodeimg").attr('src','/ValidCode.aspx?type=viewcontact&v='+Math.random());     jQuery("#view-contact").show();     var href = window.location.href;     var json = Vivian.InsertClickCompanyLink(Cid, href).value;  }  function IsTel(s) {     var patrn = /^[ /0-9-/u9fa5]*$/; ///^($\d{3,4}$|\d{3,4}-)?\d{7,8}$/;     if (!patrn.exec(s))         return false;     else         return true; }  //騾夂畑譟･逵玖＃邉ｻ蜿鷹∽ｿ｡諱ｯ function SendCompanyContactMessage(cid) { 	//$(".Wrong").hide(); 	jQuery(".view-btn button").text("謠蝉ｺ､荳ｭ...");     if(_issendcontact==false) 	{ 		return; 	} 	_issendcontact=false; 	//var cid = .trim($("#cid").val()); 	if (cid == null || cid == "" || cid == "0") { 		alert("謠千､ｺ", "", "譌豁､蜈ｬ蜿ｸ,ID髞呵ｯｯ華ƺ); 		location.reload(); 		return; 	} 	var _typename=""; 	var _ordertype="0";     var _orderflag=jQuery('input:radio[name="consultType"]:checked').val();     if(_orderflag=="1"){_typename="隸｢莉ｷ莠ｧ蜩ƺ;}     else if(_orderflag=="2"){_typename="蜚ｮ蜷取恪蜉｡";}     var title =document.getElementById("viewtitle").value;   	//var detail = title + _typename;     var detail = jQuery.trim(jQuery("#view-txtDetail").val()); 	var linkMan = document.getElementById("viewlinkname").value; // jQuery("#viewlinkname" ).val(); 	var companyName =jQuery.trim(jQuery("#viewcompanyname" ).val()); 	var tel = document.getElementById("viewtel").value;  //jQuery("#viewtel").val(); 	var email =""; 	var proID = document.getElementById("viewpid").value; //jQuery("#viewpid").val(); 	var _valCode =jQuery.trim( document.getElementById("viewcontactcode").value); //jQuery("#viewcontactcode").val(); 	var addr =""; 	var IsNoSend = jQuery('input:radio[name="isnosend4"]:checked').val();     if (typeof (IsNoSend) == "undefined") { 	    IsNoSend = "0";//鮟倩ｮ､荳崎ｽｬ蜿콻     } 	if(document.getElementById("getInfo")!=null)      {         if(document.getElementById("getInfo").checked==false)         {             IsNoSend = "0";         }	     } 	var WXTel = "";     var codename="viewcontact";  	 var    PvcID = "0"; 	 var _url=location.href; 	 var touserid="";       if (detail == "" || detail == "隸ｷ邂蜊墓緒霑ｰ謔ｨ逧콻怙豎ゑｼ碁剞200蟄ƺ) { 		_issendcontact=true; 		jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");         alert("隸ｷ蜈域緒霑ｰ謔ｨ逧콻怙豎ƺ); 		return; 	} 	else if (detail.length > 200) { 		_issendcontact=true; 		jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");         alert("謠剰ｿｰ隸ｷ謗ｧ蛻ｶ蝨ｨ200蟄嶺ｻ･蜀ƺ); 		return; 	} 	//蛹콻性  http 謌防ttps 謌ƺcom  .cn  .net  .php,.asp,.jsp 逧쓦 謠千､ｺ 	if (detail.length > 0) { 		var tempInqContent=detail.toLowerCase(); 		if ( tempInqContent.indexOf("http")>-1 || tempInqContent.indexOf("https")>-1 || tempInqContent.indexOf(".com")>-1 || tempInqContent.indexOf(".cn")>-1|| tempInqContent.indexOf(".net")>-1|| tempInqContent.indexOf(".php")>-1|| tempInqContent.indexOf(".asp")>-1|| tempInqContent.indexOf(".jsp")>-1) { 			_issendcontact=true; 			jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ"); 			alert("逡呵ｨ菫｡諱ｯ荳ｭ隸ｷ蜍ｿ蛹콻性鄂大捩華瑚ｯｷ菫ｮ謾ｹ蜷主콻谺｡謠蝉ｺ､縲ƺ);            // showInputWrong(jq("#viewtitle"),  "逡呵ｨ菫｡諱ｯ荳ｭ隸ｷ蜍ｿ蛹콻性鄂大捩華瑚ｯｷ菫ｮ謾ｹ蜷主콻谺｡謠蝉ｺ､縲ƺ); 			return; 		} 	}      if(location.href.indexOf("zyzhan")>-1 || location.href.indexOf("chem17")>-1) 	{ 		if (companyName == "" || companyName == "謔ｨ逧콻黒菴ƺ..") { 			_issendcontact=true; 			jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ"); 			 alert("隸ｷ蝪ｫ蜀呎お逧콻黒菴ƺ); 			//showInputWrong($("#viewcompanyname"), "隸ｷ蝪ｫ蜀呎お逧콻黒菴ƺ); 			return; 		} 	} 	if (linkMan == "" || linkMan == "謔ｨ逧콻ｧ灘錐...") { 		_issendcontact=true; 		jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");          alert("隸ｷ蝪ｫ蜀呎お逧콻ｧ灘錐"); 		 jQuery("#viewlinkname").focus(); 		return; 	} 	if (tel == "" || tel == "蝗ｺ螳夂鳩隸晄ƺ謇区惻...") { 		_issendcontact=true; 		jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");        alert("隸ｷ蝪ｫ蜀呵＃邉ｻ逕ｵ隸ƺ); 		jQuery("#viewtel").focus(); 	    return; 	} else {         if (!IsTel(tel)) { 			_issendcontact=true; 			jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");             alert("閨皮ｳｻ逕ｵ隸晁ｾ灘콻髞呵ｯｯ"); 			jQuery("#viewtel").focus();             return;         }     }  	if (_valCode == "") { 		_issendcontact=true; 		jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");          alert("隸ｷ蝪ｫ蜀咎ｪ瑚ｯ∫ƺ); 		 jQuery("#viewcontactcode").focus(); 		return;     } var otherInfo = ""; var isMember = "0"; var answerDate = ""; var strSex = ""; var strIndustrys = ""; var strPvc = ""; if(title=="") 	{ 		title=detail; 		if(detail.length>25) 		{ 			title=detail.substring(0,25)+"......"; 		} 	}  Vivian.NewNewSendMsgOnlySaveNoSendMeg(cid,title, proID, linkMan,companyName,tel, email,detail,   addr,_valCode,  PvcID,  _ordertype,codename, IsNoSend,"","",_orderflag,SendCompanyContactMessageCallBack);  }    function SendCompanyContactMessageCallBack(obj) {    _issendcontact=true;    jQuery(".view-btn button").text("謠蝉ｺ､菫｡諱ｯ");      var result = obj.value;  	 try {         var jobj = eval("data=" + result);         if (jobj.Detail[0].Success) {//隨ｬ荳豁･蟄伜콻謨ｰ謐ｮ蠎捺윞蜉콻             var NewOrderID = jobj.Detail[0].Success;             if (NewOrderID >0) { 			    Vivian.SendMsgOnlySaveNoSendMeg_CallBackToSend(NewOrderID, function() { return false; });                   jQuery("#view-contact").hide(); 				// jQuery("#view-contact-success").show(); 				//alert("謔ｨ逧・蕗險蟾ｲ謠蝉ｺ､謌仙粥華∵콻莉ｬ蟆콻惠隨ｬ荳譌ｶ髣ｴ蝗槫､肴お~"); 				jQuery("#viewcompanyname").val(''); 				jQuery("#viewlinkname").val(''); 				jQuery("#viewtel").val(''); 				jQuery("#viewcontactcode").val(''); 				var man=jQuery("#viewlinkname").val(''); 				var _proid=jQuery("#viewpid").val(); 				if(location.href.indexOf("sale")>-1) 					Vivian.ReCompanyMobileBySID(_proid,GetMobileCallBack); 				else 					Vivian.ReCompanyMobileByPID(_proid,GetMobileCallBack);             }             else {                  switch (NewOrderID) { 					case -1:                         alert("鬪瑚ｯ∫∝､ｱ謨茨ｼ∬ｯｷ轤ｹ蜃ｻ鬪瑚ｯ∫∬ｿ幄｡悟姐譁ｰ謫堺ｽ懶ｼƺ);                         break;                     case -5:                         alert("蠕域干豁会ｼ梧お逧안ｿ｡諱ｯ蛹콻性莠콻撼豕募ｭ礼ｬｦ(" + jsonobj.Detail[0].DirtyWords + ")華瑚ｯｷ菫ｮ謾ｹ華ƺ);                         break; 					 case -10:                         alert("逡呵ｨ謌仙粥華碁ぐ莉ｶ蜿鷹∝､ｱ雍･華ƺ);                         break; 					case -11: 						alert("逡呵ｨ菫｡諱ｯ荳ｭ隸ｷ蜍ｿ蛹콻性鄂大捩華瑚ｯｷ菫ｮ謾ｹ蜷主콻谺｡謠蝉ｺ､華ƺ); 						break;                     case -2016001:                         alert("螟ｱ雍･華∵お蜿鷹∽ｿ｡諱ｯ螟ｪ霑콻｢醍ｹ・ｼƺ);                         break;                     case -2016002:                         alert("螟ｱ雍･華∫罰莠取お譖ｾ鬚醍ｹ∝書騾！P蟾ｲ扈剰｢ｫ蟆콻煤華ƺ);                         break; 					default: 					    alert("謨ｰ謐ｮ蜀吝콻螟ｱ雍･");                         break;                  }             }         }         else {             alert("逡呵ｨ螟ｱ雍･");         }     }     catch (ex) {         alert("逡呵ｨ螟ｱ雍･縲ƺ);     }  jQuery("#viewcodeimg").attr('src','/ValidCode.aspx?type=viewcontact&v='+Math.random()); } function GetMobileCallBack(obj) { 	jQuery("#view-contact-success").show();     var result = obj.value; 	if(result!=null && result!="") 				jQuery("#viewcontentmobile").html(result); 		} </script>  <script>     jQuery(document).ready(function () {         var chathm = document.createElement("script");         chathm.src = "https://chat.gsrxyx.com/chat/KFRightBox/146609";         document.head.appendChild(chathm);          var chathm2 = document.createElement("script");         chathm2.src = "https://chat.gsrxyx.com/chat/KFCenterBox/146609";         document.head.appendChild(chathm2);     })      </script> <div style="display:none">   	</div> <link rel='stylesheet' type='text/css' href='/css/xwt-notice.css?v=1111' />                                     <link rel='stylesheet' type='text/css' href='/Font/font_2941626/iconfont.css' />                                     <script type='text/javascript' src='/JavaScript/soundmanager2-nodebug-jsmin.js'></script>                                     <script>!window.jQuery && document.write('<script src=\'https://public.mtnets.com/Plugins/jQuery/2.2.4/jquery-2.2.4.min.js\'>'+'</scr'+'ipt>');</script>                                     <script type='text/javascript' src='/js/XwtEnclosure.js?v=202506070900'></script>  <script type='text/javascript' src='/js/AdvertiseTotle.js?v=202506070900'></script>    </body> </html>

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